Bonnie Bassler: The secret, social lives of bacteria

翻译人员: Zhenyu Zhou 校对人员: elyse lin

细菌是地球上最古老的生物.

Bacteria are the oldest living organisms on the earth.

They've been here for billions of years,

它们已经存在数十亿年了

and what they are are single-celled microscopic organisms.

它们是单细胞微生物

So they're one cell

它们特征是只有一个细胞

and they have this special property that they only have one piece of DNA.

还有就是它们只有一份DNA

So they have very few genes and genetic information

它们只有少量基因,

和遗传信息来编码它们表达的特性。

to encode all of the traits that they carry out.

And the way bacteria make a living is that they consume nutrients

细菌生存的方法

是不断从环境中吸取养分©

from the environment,

they grow to twice their size,

在成长到两倍的体积后，它们从中一分为二

they cut themselves down in the middle,

分裂为两个细胞，如此循环

and one cell becomes two, and so on and so on.

They just grow and divide and grow and divide -- so a kind of boring life,

它们不停得生长、分裂，然后再生长、再分裂—过着有点乏味的生活。

except that what I would argue is that you have an amazing interaction

但是，今天我想告诉你

你与这些细菌有着惊人的互动关系

with these critters.

I know you guys think of yourself as humans,

我知道你认为你自己是人类，而这可能也是我如何看你们的

and this is sort of how I think of you.

在这里的是

This man is supposed to represent a generic human being,

一个一般人类的代表

and all of the circles in that man are all the cells that make up your body.

在他身上所有的圆圈代表着各个组成人体的细胞

There's about a trillion human cells that make each one of us who we are

每个人体大约是由一兆个人体细胞所组成

它们让我们能完成各种各样我们想做的事情

and able to do all the things that we do.

但是，你一生中的每时每刻，

But you have 10 trillion bacterial cells in you or on you

有大约十兆个细菌细胞生活在你的体内体表。

at any moment in your life.

So, 10 times more bacterial cells than human cells on a human being.

所以，有十倍于人体细胞的细菌细胞

生活在一个人身上

And, of course, it's the DNA that counts,

同理, 我们要算一下DNA

so here's all the A, T, Gs and Cs that make up your genetic code

这是所有的 A, T, G 和 C (腺嘌呤, 胸腺嘧啶, 鸟嘌呤, 胞嘧啶)

组成你的基因密码, 赋予你所有的魅力特征.

and give you all your charming characteristics.

你有3万左右的遗传基因,

You have about 30,000 genes.

Well, it turns out you have 100 times more bacterial genes

而围绕你的细菌的遗传基因数量是你自己的100倍

它们在你的身体内部和表面上中始终扮演着重要的角色。

playing a role in you or on you all of your life.

So at the best, you're 10 percent human; more likely, about one percent human,

最乐观的看法是: 你只是"10分之1人",

事实上"100分之一人"更准确，

depending on which of these metrics you like.

取决于你更喜欢用哪个尺度来衡量.

I know you think of yourself as human beings,

我知道你自认为是一个"人类",

但在我眼里你是90%~99%的细菌.

but I think of you as 90 or 99 percent bacterial.

(Laughter)

(笑)

And these bacteria are not passive riders.

这些细菌不是顺从的乘客,

These are incredibly important; they keep us alive.

他们难以置信得重要, 他们让我们活着.

They cover us in an invisible body armor

它们是我们身上的无形盔甲

that keeps environmental insults out so that we stay healthy.

阻断来之环境的伤害

保持我们的健康.

They digest our food, they make our vitamins,

它们消化食物, 制造维他命,

they actually educate your immune system to keep bad microbes out.

它们还指导你的免疫系统

将有害微生物阻挡在体外。

它们尽心尽职干活

So they do all these amazing things

帮助我们，维护我们的生命。

that help us and are vital for keeping us alive,

and they never get any press for that.

却从没有因此得到过报道.

But they get a lot of press because they do a lot of terrible things as well.

反而却因为它们同时做的许多

坏事而时常见报。

地球上有无数种细菌

So there's all kinds of bacteria on the earth

that have no business being in you or on you at any time,

它们有些在任何时候都绝对不应该出现在你的体内体表，

然而假如你不幸遇到了, 那你一定会病得很厉害。

and if they are, they make you incredibly sick.

And so the question for my lab

所以, 我们实验室研究的问题是, 正是你想知道的

is whether you want to think about all the good things that bacteria do

细菌做的所有好事或者细菌做的所有坏事。

or all the bad things that bacteria do.

The question we had is: How could they do anything at all?

我们曾经提出一个疑问: 它们究竟是怎么做到的?

我指的是, 它们是那么细微,

I mean, they're incredibly small.

You have to have a microscope to see one.

用显微镜才能看到一个。

They live this sort of boring life where they grow and divide,

它们的生活好像只是单调乏味的成长与分裂，

and they've always been considered to be these asocial, reclusive organisms.

而且长久以来被认为是不善社交的隐居生命体。

And so it seemed to us that they're just too small

所以在我们看来, 它们实在是太渺小，

to have an impact on the environment

如果单枪匹马

if they simply act as individuals.

根本到无法对环境产生任何影响。

So we wanted to think if there couldn't be a different way that bacteria live.

所以我们正在探讨

细菌是不是有着特殊的生存方式。

解答这个问题的线索

And the clue to this came from another marine bacterium,

and it's a bacterium called "Vibrio fischeri."

来自一种叫做费氏弧菌的海洋细菌。

What you're looking at on this slide is just a person from my lab

你们在这张幻灯看到的，是我实验室的一个工作人员

holding a flask of a liquid culture of a bacterium,

握着一瓶装满这种细菌的培养液，

这是一种来自海洋的，美丽而且无害的细菌：

a harmless, beautiful bacterium that comes from the ocean,

"费氏弧菌".

named Vibrio fischeri.

And this bacterium has the special property that it makes light,

这种细菌的特性是会发光，

产生生物荧光,

so it makes bioluminescence,

like fireflies make light.

像萤火虫一样。

We're not doing anything to the cells here,

我们没有对这些细胞做任何处理。

we just took the picture by turning the lights off in the room,

我们只是把房间灯关了，然后照了这张照片，

这是我们所见到的情形。

and this is what we see.

And what's actually interesting to us was not that the bacteria made light

事实上, 我们感兴趣的部分

并不是细菌会不会发光，

but when the bacteria made light.

而是细菌何时发光。

What we noticed is when the bacteria were alone,

我们发现, 当细菌独立存在,

so when they were in dilute suspension,

经稀释后进行悬浮培养时, 它们不发光。

they made no light.

但一旦它们增长到一个特定数量之后

But when they grew to a certain cell number,

所有的细菌同时发光。

all the bacteria turned on light simultaneously.

So the question that we had is:

于是我们想, 这些原始生物,

How can bacteria, these primitive organisms,

到底如何得知自己是处于孤立的状态,

tell the difference from times when they're alone

还是处在一个群体里，

and times when they're in a community,

并且同时一起做同一件事情。

and then all do something together?

然后我们发现了这是因为细菌能够彼此交谈，

And what we figured out is that the way they do that is they talk to each other,

它们用的是化学语言。

and they talk with a chemical language.

假设这是我的细菌。

So this is now supposed to be my bacterial cell.

当它独处时, 丝毫不会发光。

When it's alone, it doesn't make any light.

But what it does do is to make and secrete small molecules

但是它们会制造分泌小化学分子

that you can think of like hormones,

你可以把他想象成荷尔蒙,

and these are the red triangles.

这里的红色三角形代表这些小分子, 当细菌独处时，

And when the bacteria are alone, the molecules just float away,

分泌的小分子游离开来,所以不发光。

and so, no light.

But when the bacteria grow and double

随着这些细菌成倍增长,

and they're all participating in making these molecules,

且全部一起制造这些分子,

这些细胞外分子的含量

the molecule, the extracellular amount of that molecule,

increases in proportion to cell number.

随着细胞数量的增加而增加，

And when the molecule hits a certain amount

当这些个分子累积到一定的量之后，

that tells the bacteria how many neighbors there are,

它们会告诉了细菌，它们周围有多少邻居，

they recognize that molecule

细菌接受到这些信息后，

and all of the bacteria turn on light in synchrony.

所有的细菌，同时开始发光。

And so that's how bioluminescence works --

生物光就是这样运作的 --

they're talking with these chemical words.

它们籍由上述的化学语言交流。

费氏弧菌的发光现象来自生物学上的原因。

The reason Vibrio fischeri is doing that comes from the biology --

again, another plug for the animals in the ocean.

接下来,我们再来看一个海洋生物：

费式弧菌寄生在这种乌贼的体内。

Vibrio fischeri lives in this squid.

What you're looking at is the Hawaiian bobtail squid.

你们现在看到的是夏威夷截尾乌贼,

这是它的腹侧,

It's been turned on its back,

and what I hope you can see are these two glowing lobes.

我希望你们看得到，那两个发着光的叶状突起，

These house the Vibrio fischeri cells.

这里是费式弧菌的寄生之处，

They live in there, at high cell number.

他们居住在这里面。

That molecule is there, and they're making light.

他们分泌的小分子也在这里面,所以它们能够发光。

这种乌贼之所以愿意接受它们在里面胡作非为

And the reason the squid is willing to put up with these shenanigans

是因为它需要这些亮光。

is because it wants that light.

这种共存行为的建立基础

The way that this symbiosis works

是因为这种小乌贼生活在夏威夷的海岸，

is that this little squid lives just off the coast of Hawaii,

just in sort of shallow knee-deep water.

大概只有膝盖一般深的水里。

And the squid is nocturnal,

这种乌贼是夜行性的，

so during the day, it buries itself in the sand and sleeps.

因此在白天它们藏在沙子里睡觉，

但是到了晚上，它们必须出来猎食。

But then at night, it has to come out to hunt.

So on bright nights

在有着许多星光与月光的明亮夜晚，

when there's lots of starlight or moonlight,

这些光线可以照透乌贼所生活的地方

that light can penetrate the depth of the water the squid lives in,

因为这里的海水只有数尺深而已

since it's just in those couple feet of water.

这种乌贼演化出了一种活叶瓣，

What the squid has developed is a shutter that can open and close

可以打开或关闭细菌所寄生的发光器官。

over the specialized light organ housing the bacteria.

这种乌贼背上有一些感光装置，

And then it has detectors on its back

可以测量有多少月光或星光照在它背上，

so it can sense how much starlight or moonlight is hitting its back.

And it opens and closes the shutter

然后调节它的活叶瓣。

so the amount of light coming out of the bottom,

使从它腹部所放出的光

which is made by the bacterium,

细菌产生的光

exactly matches how much light hits the squid's back,

完全符合照射在乌贼背上的光强度

so the squid doesn't make a shadow.

因此这乌贼就不会产生任何影子。

So it actually uses the light from the bacteria

它们使用来自细菌的光，

to counter-illuminate itself in an antipredation device,

不断调节光线，就像穿上隐身衣，

使猎食者无法看见它的阴影，

so predators can't see its shadow,

计算它的动向，然后吃了它。

calculate its trajectory and eat it.

就像是大海中的隐形轰炸机一般。

So this is like the stealth bomber of the ocean.

(Laughter)

（笑声）

But then if you think about it, this squid has this terrible problem,

但是如果你再深入地想一下，这乌贼会有一个可怕的问题

因为在它的体内，这些黏稠的细菌会不断的增长，死亡，

because it's got this dying, thick culture of bacteria,

and it can't sustain that.

乌贼无法无限地维持这种状态。

And so what happens is, every morning when the sun comes up,

因此每天早上太阳升起后，

它将自己埋藏在沙子里睡觉，

the squid goes back to sleep, it buries itself in the sand,

它有一个与日夜周期同步的活泵

and it's got a pump that's attached to its circadian rhythm.

当太阳升起时，它将大约95%的细菌排出体外。

And when the sun comes up, it pumps out, like, 95 percent of the bacteria.

So now the bacteria are dilute,

当细菌被稀释了，这些小荷尔蒙分子也随之消失，

that little hormone molecule is gone, so they're not making light.

因此它们就不发光了，

But, of course, the squid doesn't care, it's asleep in the sand.

但这时乌贼完全不在乎，因为它正在沙子里睡觉呢。

当白天过去，这些细菌持续分裂增长，

And as the day goes by, the bacteria double,

它们释放出足够的这些小分子，然后又开始在晚上发光，

they release the molecule, and then light comes on at night,

这时正好又是乌贼需要光线的时候。

exactly when the squid wants it.

So first, we figured out how this bacterium does this,

我们首先了解了这些细菌为什么会有这种现象，

but then we brought the tools of molecular biology to this

然后我们使用分子生物学的方法

to figure out, really, what's the mechanism.

来研究这种现象的真正的机理。

And what we found -- so this is now supposed to be my bacterial cell --

我们发现了：比如这是我的费氏弧菌

is that Vibrio fischeri has a protein.

它有一种蛋白质－

That's the red box --

就是这个红色的方块—它是一种催化剂，

it's an enzyme that makes that little hormone molecule,

是它制造的这种小荷尔蒙分子，就是这红色三角形。

the red triangle.

And then as the cells grow,

当细胞生长时，他们全都释放这个种分子到环境中，

they're all releasing that molecule into the environment,

因此周围环境中有大量的这种分子。

so there's lots of molecule there.

这些细菌的细胞表面，存在一种受体，

And the bacteria also have a receptor on their cell surface

that fits like a lock and key with that molecule.

它与小分子的构造就如同锁与钥匙一般的吻合。

These are just like the receptors on the surfaces of your cells.

它们就如同你我身体细胞表面上的受体一般。

当这些分子增加到一定的量时—

So when the molecule increases to a certain amount,

which says something about the number of cells,

这也意味着这些细胞的数量增加到一定的量

it locks down into that receptor

荷尔蒙小分子与受器相结合，

and information comes into the cells

讯息开始向细胞内部传递，

这个讯息会告诉这些细胞

that tells the cells to turn on this collective behavior of making light.

开始集体发光的行为。

Why this is interesting is because in the past decade,

这个发现之所以有趣，是因为在过去十年间

we have found that this is not just some anomaly

我们发现这种现象，

of this ridiculous, glow-in-the-dark bacterium that lives in the ocean --

不只局限在这种住在大海中，古怪的、会在黑暗中发光的细菌，

而是所有的细菌都有类似的系统。

all bacteria have systems like this.

So now what we understand is that all bacteria can talk to each other.

由此，我们发现所有细菌都是可以彼此交谈的。

They make chemical words, they recognize those words,

它们制造化学文字，同时也能够辨认这些文字，

and they turn on group behaviors

然后表现

that are only successful when all of the cells participate in unison.

只有当所有细胞齐心协力才能成功的集体行为。

So now we have a fancy name for this: we call it "quorum sensing."

我们为这种行为取了一个新潮的名字，称作：聚量感应。

They vote with these chemical votes,

就象用化学物质投票，

the vote gets counted, and then everybody responds to the vote.

再对票量加以统计，然后 所有细胞都要服从最后的投票结果。

What's important for today's talk is we know there are hundreds of behaviors

今天演讲最重要的一点是，

我们已经知道细菌有数百种以上的这种

that bacteria carry out in these collective fashions.

集体行为。

But the one that's probably the most important to you is virulence.

其中对大家来说，最关心的应该还是细菌的致病性。

并不是说少量细菌进入你体内后

It's not like a couple bacteria get in you and start secreting some toxins --

就马上开始分泌毒素。

you're enormous; that would have no effect on you, you're huge.

相对它们来说，你是个庞然大物，少量细菌对你不会有任何的影响。

我们发现，

But what they do, we now understand,

is they get in you, they wait, they start growing,

它们是先进入你的体内，然后等待，开始不断复制增长，

they count themselves with these little molecules,

它们由统计小分子的数目来估计自身的实力，

and they recognize when they have the right cell number

当确定有足够的细胞数后，

所有细菌一起发动致病攻击，

that if all of the bacteria launch their virulence attack together,

they're going to be successful at overcoming an enormous host.

这样它们就能成功攻陷巨大的宿主。

So bacteria always control pathogenicity with quorum sensing.

细菌总是以「聚量感应」来控制其致病性

这就是它们运作的原理。

So that's how it works.

我们同时也研究了这些小分子，

We also then went to look at what are these molecules.

These were the red triangles on my slides before.

这些就是我刚才幻灯上的小红三角形。

This is the Vibrio fischeri molecule.

这个是费氏弧菌的小分子。

This is the word that it talks with.

也就是它们用以交谈的文字。

And then we started to look at other bacteria,

我们开始研究其他细菌，

and these are just a smattering of the molecules that we've discovered.

这些是部份我们已发现小分子

我希望你们看得出来

What I hope you can see is that the molecules are related.

这些分子之间是有关联性的。

The left-hand part of the molecule is identical

每种细菌，

in every single species of bacteria.

它们的小分子的左半部都是完全相同的。

But the right-hand part of the molecule is a little bit different

只是在右半部则因菌种的不同而有少许的不同。

in every single species.

这个发现证实

What that does is to confer exquisite species specificities to these languages.

细菌的语言有高度的专一性。

So each molecule fits into its partner receptor

每一种分子只能与其相对受器结合，非常专一。

and no other.

所以这些交谈是私下的、秘密的。

So these are private, secret conversations.

These conversations are for intraspecies communication.

这种交流是只限于同种族内部的沟通。

Each bacteria uses a particular molecule that's its language

每一种细菌使用其特殊分子代表它的语言，

that allows it to count its own siblings.

让它能够计算同类的数量。

一旦我们了解这些，

Once we got that far,

we thought we were starting to understand that bacteria have these social behaviors.

我们也开始了解细菌有所谓的社交行为。

但我们真正思考的问题是，

But what we were really thinking about is that most of the time,

在多数情况下，细菌并不是单独生活的，它们居住的地方是鱼龙混杂的，

bacteria don't live by themselves, they live in incredible mixtures,

它们跟其它成百上千种的细菌同居一处。

with hundreds or thousands of other species of bacteria.

And that's depicted on this slide.

这张幻灯可以说明这个情形：这是你的皮肤。

This is your skin.

So this is just a picture -- a micrograph of your skin.

这是一张照片，是你皮肤的显微照片。

Anywhere on your body, it looks pretty much like this.

在你身体的任何地方，看上去都会和这差不多。

我希望你能看出，这里有各种不同的细菌。

What I hope you can see

is that there's all kinds of bacteria there.

And so we started to think, if this really is about communication in bacteria,

因此我们开始思考，如果这真的是细菌间的交流

and it's about counting your neighbors,

计算与之相邻的同种细菌的数量，

it's not enough to be able to only talk within your species.

只跟同种细菌沟通是不够的，

它们一定有某种

There has to be a way to take a census

跟周围其他种细菌和平共处的方法。

of the rest of the bacteria in the population.

所以我们回到分子生物学的方法，

So we went back to molecular biology

开始研究不同的细菌。

and started studying different bacteria.

我们现在已经发现，

And what we've found now is that, in fact, bacteria are multilingual.

事实上，细菌可以讲多国种语言。

它们都有一个菌种特别识别系统，

They all have a species-specific system,

用特别分子来辨别同类。

they have a molecule that says "me."

But then running in parallel to that is a second system

但是，我们发现，

that we've discovered, that's generic.

它们同时还有另一种系统，那是一个通用的系统。

So they have a second enzyme that makes a second signal,

因此，它们有另一种催化剂，能产生第二种信号，

and it has its own receptor,

这种信号也有自己的受体，

and this molecule is the trade language of bacteria.

这种分子是细菌们的公共语言。

它被所有不同的细菌所公共使用，

It's used by all different bacteria,

是一种菌种间沟通的语言。

and it's the language of interspecies communication.

What happens is that bacteria are able to count

细菌能够计算

并区分自己周围同种与异种细菌的数量。

how many of "me" and how many of "you."

And they take that information inside,

它们传递这些讯息到细胞内，

and they decide what tasks to carry out

然后决定该怎么做，

它们的行动取决于在整个群体中

depending on who's in the minority and who's in the majority

of any given population.

谁占多数，谁占少数。

Then, again, we turned to chemistry,

我们又使用化学方法

and we figured out what this generic molecule is --

搞清了这个通用分子的构造－

that was the pink ovals on my last slide, this is it.

通用分子就是我上一张幻灯的粉红色椭圆形。

It's a very small, five-carbon molecule.

它是一个非常小的五碳分子。

重要的是，我们发现

And what the important thing is that we learned

is that every bacterium has exactly the same enzyme

每种细菌都有完全一样的催化剂，

and makes exactly the same molecule.

可以制造一模一样的分子。

So they're all using this molecule for interspecies communication.

它们都使用这个分子

作为菌种间交流的语言。

This is the bacterial Esperanto.

这是细菌的世界语。

(Laughter)

笑声

So once we got that far,

一旦我们了解这个后，

we started to learn that bacteria can talk to each other

我们知道细菌可以用这个分子来相互交流。

with this chemical language.

但是我们又开始思考，

But we started to think

that maybe there is something practical that we can do here as well.

也许我们可以使用这个发现来做一些实质上的应用。

我已经告诉过你们，细菌间是有社交行为的，

I've told you that bacteria have all these social behaviors,

它们是使用这些分子进行交流的。

that they communicate with these molecules.

Of course, I've also told you that one of the important things they do

当然，我也告诉过你，

其中一件主要的事情就是它们使用聚量感应来启动致病性。

is to initiate pathogenicity using quorum sensing.

我们不禁想，我们是不是

So we thought:

What if we made these bacteria so they can't talk or they can't hear?

可以让这些细菌哑了或聋了？

这岂不是可以成为一种新的抗生素？

Couldn't these be new kinds of antibiotics?

And of course, you've just heard and you already know

当然，你一定听说过，而且你早就知道了

我们快要没有有效的抗生素了。

that we're running out of antibiotics.

现在的细菌都拥有，不可思议的多重耐药性

Bacteria are incredibly multi-drug-resistant right now,

and that's because all of the antibiotics that we use kill bacteria.

而这都是因为，这些抗生素的工作原理都是杀死细菌。

They either pop the bacterial membrane,

它们要么是使细菌的细胞膜破裂，

they make the bacterium so it can't replicate its DNA.

就是不让细菌复制自己的DNA。

We kill bacteria with traditional antibiotics,

当我们用传统抗生素来杀菌时，

and that selects for resistant mutants.

会同时培养筛选出有耐药性的突变菌株。

And so now, of course, we have this global problem

所以，我们有了全球性的

in infectious diseases.

感染病问题。

So we thought, what if we could sort of do behavior modifications,

我们想，如果我们可以稍微更改这些细菌的行为，

just make these bacteria so they can't talk, they can't count,

只是让这些细菌无法交谈，无法计数，

它们就不知何时发起毒性攻击。

and they don't know to launch virulence?

So that's exactly what we've done,

这就是我们已经完成的实验，我们使用了两种不同策略：

and we've sort of taken two strategies.

第一个，

The first one is, we've targeted the intraspecies communication system.

我们锁定菌种内通讯系统。

So we made molecules that look kind of like the real molecules, which you saw,

我们制造了一些看起来跟真的分子很像的分子，

你们可以看到，它们间只有一点点的不同。

but they're a little bit different.

因此，它们会锁住这些受体，

And so they lock into those receptors,

并且干扰受体辨识真正的分子。

and they jam recognition of the real thing.

So by targeting the red system,

既然锁定红色的系统，

what we are able to do is make species-specific, or disease-specific,

我们就可以制造

针对菌种，或是针对疾病的「反聚量感应」分子。

anti-quorum-sensing molecules.

We've also done the same thing with the pink system.

我们也对粉红色系统做了同样的事情。

We've taken that universal molecule and turned it around a little bit

我们使用那种通用分子，将之做了一些更改

so that we've made antagonists of the interspecies communication system.

我们做了一些拮抗剂，

它们都是针对菌种间的通讯系统。

我们希望这些分子可以用来作广谱抗生素，

The hope is that these will be used as broad-spectrum antibiotics

that work against all bacteria.

对所有细菌都有效。

And so to finish, I'll show you the strategy.

最后，我只跟你们说一下战略。

In this one, I'm just using the interspecies molecule,

在这个里，我们只是使用跨菌种分子，

but the logic is exactly the same.

但是思维逻辑是一模一样的。

So what you know is that when that bacterium gets into the animal --

你们都知道，当细菌进入动物体内，

以此为例，对这只老鼠，

in this case, a mouse --

it doesn't initiate virulence right away.

它并不会马上启动致病机制。

It gets in, it starts growing,

它进入，开始增殖，

it starts secreting its quorum-sensing molecules.

开始分泌它的聚量感应分子。

It recognizes when it has enough bacteria

当累积到足够数量时，细菌能识别，

that now they're going to launch their attack,

并开始发起攻击，

然后老鼠就死了。

and the animal dies.

And so what we've been able to do is to give these virulent infections,

我们所做的是在致病感染的同时，

but we give them in conjunction with our anti-quorum-sensing molecules.

加入我们的「反聚量感应分子」，

So these are molecules that look kind of like the real thing,

也就是看起来很像真的「聚量感应分子」的物质，

但是，正如同我在幻灯上指出的，它们之间有一点点不同。

but they're a little different, which I've depicted on this slide.

我们现在发现，

What we now know is that if we treat the animal with a pathogenic bacterium --

如果让实验动物感染一种具有多重耐药性的致病细菌，

a multi-drug-resistant pathogenic bacterium --

in the same time we give our anti-quorum-sensing molecule,

但是同时， 我们施予「反聚量感应分子」治疗，

in fact, the animal lives.

动物就能够存活。

And so we think that this is the next generation of antibiotics,

我们认为这应该是下一代的抗生素，

and it's going to get us around, at least initially,

它将能够让我们，至少在开始阶段，

this big problem of resistance.

解决耐药性细菌的问题。

What I hope you think is that bacteria can talk to each other,

我希望你们也能认为，细菌可以彼此交谈，

它们使用化学物质当作语言，

they use chemicals as their words,

they have an incredibly complicated chemical lexicon

它们拥有极端复杂的化学语汇，

that we're just now starting to learn about.

我们现在才刚刚要开始学习这些语汇。

当然，也因为这些语汇

Of course, what that allows bacteria to do is to be multicellular.

使细菌得以变得像多细胞生物。

So in the spirit of TED,

所以，就像TED的精神一样，它们彼此合作，

they're doing things together because it makes a difference.

因为这样才能有一番作为。

细菌因为有这些集体行为，

What happens is that bacteria have these collective behaviors,

and they can carry out tasks

所以可以执行

that they could never accomplish if they simply acted as individuals.

一些如果是单枪匹马

是永远无法完成的任务。

What I would hope that I could further argue to you

我希望能进一步地说服你们的是

这就是多细胞生物的起源。

is that this is the invention of multicellularity.

Bacteria have been on the earth for billions of years;

细菌已经生存在地球上数十亿年了。

humans, couple hundred thousand.

人类只有数十万年而已。

So we think bacteria made the rules for how multicellular organization works.

我们认为细菌制定了

多细胞的组织运作规则。

我们认为，由研究细菌，

And we think by studying bacteria,

we're going to be able to have insight about multicellularity in the human body.

我们将能够对，人体内的多细胞系统，有更进一步的认识。

So we know that the principles and the rules,

我们现在已经知道基本规则了，

if we can figure them out in these sort of primitive organisms,

如果我们可以从这些原始生命体上进一步弄懂它们，

这些规则也有希望能够应用

the hope is that they will be applied

到人类其它疾病与行为上。

to other human diseases and human behaviors as well.

我希望你们已经学到

I hope that what you've learned

is that bacteria can distinguish self from other.

细菌是可以区分你我，

So by using these two molecules,

使用这两种分子，它们可以表达「我」和「你」。

they can say "me" and they can say "you."

当然，这就是我们所做的

And again, of course, that's what we do,

不仅只在分子层面上，

both in a molecular way, and also in an outward way,

同样也在行为上，

but I think about the molecular stuff.

只是我在分子层次上想的多一点。

This is exactly what happens in your body.

这完全就是你们体内正在发生的事情。

It's not like your heart cells and kidney cells get all mixed up every day,

你们的心脏和肾脏细胞不会每天混杂在一起，

这是因为你体内有一大堆化学反应不断地进行着，

and that's because there's all of this chemistry going on,

these molecules that say who each of these groups of cells is

这些分子能够区分不同的细胞群组，

还有它们所应该执行的任务。

and what their tasks should be.

So again, we think bacteria invented that,

再一次，我们认为细菌发明了这个机制，

你只不过是多演化出了一些铃铛与哨子而已，

and you've just evolved a few more bells and whistles,

but all of the ideas are in these simple systems that we can study.

但是所有的概念都包含在这个我们所研究的简单系统中。

And the final thing is, just to reiterate that there's this practical part,

最后，只是再一次重申，这个研究的实际应用方面，

and so we've made these anti-quorum-sensing molecules

是我们已经制造出了这些「反聚量感应分子」，

that are being developed as new kinds of therapeutics.

它们正作为新一代的疗法被开发研究中。

But then, to finish with a plug for all the good and miraculous bacteria

为了鼓励地球上生存的

that live on the earth,

所有对人有益的细菌，

we've also made pro-quorum-sensing molecules.

我们也制造了「强化聚量感应分子」。

So we've targeted those systems to make the molecules work better.

因此，我们已经锁定了这些系统，让这些分子运作得更好。

请记得在你体内体表，有超过你自身细胞十倍的细菌，

So remember, you have these 10 times or more bacterial cells

in you or on you, keeping you healthy.

它们使你保持健康。

What we're also trying to do is to beef up the conversation

我们也努力加强

你和你的共生的细菌之间交流，

of the bacteria that live as mutualists with you,

in the hopes of making you more healthy,

让你更健康，

让这种互动更有益。

making those conversations better,

so bacteria can do things that we want them to do

让细菌只做我们希望它们做的事情。

better than they would be on their own.

防止细菌做我们不希望它们做的事情。

Finally, I wanted to show you --

最后，我希望让你们看看

this is my gang at Princeton, New Jersey.

我在新泽西，普林斯顿大学实验室的成员。

我今天所告诉你们的每一个发现，都是由照片中的某人所完成的。

Everything I told you about was discovered by someone in that picture.

我希望当你们学到东西的同时

And I hope when you learn things, like about how the natural world works --

比如：自然世界是怎样运作的，

I just want to say that whenever you read something in the newspaper

我只是想说，任何时候，当你们在报纸上看到某事，

or you hear some talk about something ridiculous in the natural world,

或是你们听到某些有关自然的，好玩事情的演讲，

都是由年轻人完成的。

it was done by a child.

So science is done by that demographic.

科学是由这种年龄层的人所造就的。

这些二、三十岁的年轻人

All of those people are between 20 and 30 years old,

and they are the engine that drives scientific discovery in this country.

他们是推动这个国家科学发现的引擎。

我真的是非常幸运能与这群年轻人一起共事。

And it's a really lucky demographic to work with.

(Applause)

我自己在不断地变老，但他们却是始终不变，

I keep getting older and older, and they're always the same age.

这真是一个美好得不能再好的工作。

And it's just a crazy, delightful job.

And I want to thank you for inviting me here,

我要谢谢你们的邀请。

it's a big treat for me to get to come to this conference.

非常荣幸能参加这个大会。

(Applause)

掌声

Thanks.

谢谢

(Applause)

掌声